CRCST Practice Exam 2026 – Complete Study Resource

Question: 1 / 400

What steps should be taken if a biological indicator does not show a negative result?

Ignore the result and proceed with use

Investigate the cause and reprocess the affected items

When a biological indicator does not show a negative result, it signifies that the sterilization process may not have been successful, and viable microorganisms may still be present on the items being monitored. Taking steps to investigate the cause and reprocess the affected items is crucial for patient safety and infection control.

Reprocessing the items ensures that any potential contamination is eliminated, effectively adhering to the standards of practice within sterilization procedures. This action also involves reviewing the sterilization process used, checking equipment functionality, and ensuring that the correct parameters were met during the cycle. This thorough investigation not only protects patients who may receive the items but also prevents potential outbreaks of infections linked to inadequate sterilization practices.

Other responses might suggest ignoring the result, reporting it only to maintenance, or merely documenting the event without further action. These approaches do not adequately address the potential risks associated with failing to properly ensure that sterilization has been successful and compromise patient safety. Therefore, investigating the cause and reprocessing is the most responsible and necessary step in ensuring quality control and safety in medical environments.

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Report it to the maintenance team only

Document the event without further action

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